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Comparative study of “Commercially available mouthwashes with that of herbal mouthwashes” on pre-dominant oral cavity flora- Streptococcus species

M.K. Esha1 , K. Anuradha2

Section:Research Paper, Product Type: Journal Paper
Vol.06 , Special Issue.01 , pp.25-30, May-2019

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6si1.2530

Online published on May 10, 2019

Copyright M.K. Esha, K. Anuradha . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.  

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IEEE Style Citation: M.K. Esha, K. Anuradha, Comparative study of “Commercially available mouthwashes with that of herbal mouthwashes” on pre-dominant oral cavity flora- Streptococcus species, International Journal of Scientific Research in Biological Sciences, Vol.06, Issue.01, pp.25-30, 2019.

MLA Style Citation: M.K. Esha, K. Anuradha "Comparative study of “Commercially available mouthwashes with that of herbal mouthwashes” on pre-dominant oral cavity flora- Streptococcus species." International Journal of Scientific Research in Biological Sciences 06.01 (2019): 25-30.

APA Style Citation: M.K. Esha, K. Anuradha, (2019). Comparative study of “Commercially available mouthwashes with that of herbal mouthwashes” on pre-dominant oral cavity flora- Streptococcus species. International Journal of Scientific Research in Biological Sciences, 06(01), 25-30.

BibTex Style Citation:
@article{Esha_2019,
author = {M.K. Esha, K. Anuradha},
title = {Comparative study of “Commercially available mouthwashes with that of herbal mouthwashes” on pre-dominant oral cavity flora- Streptococcus species},
journal = {International Journal of Scientific Research in Biological Sciences},
issue_date = {5 2019},
volume = {06},
Issue = {01},
month = {5},
year = {2019},
issn = {2347-2693},
pages = {25-30},
url = {https://www.isroset.org/journal/IJSRBS/full_paper_view.php?paper_id=23},
doi = {https://doi.org/10.26438/ijcse/v6i1.2530}
publisher = {IJCSE, Indore, INDIA},
}

RIS Style Citation:
TY - JOUR
DO = {https://doi.org/10.26438/ijcse/v6i1.2530}
UR - https://www.isroset.org/journal/IJSRBS/full_spl_paper_view.php?paper_id=23
TI - Comparative study of “Commercially available mouthwashes with that of herbal mouthwashes” on pre-dominant oral cavity flora- Streptococcus species
T2 - International Journal of Scientific Research in Biological Sciences
AU - M.K. Esha, K. Anuradha
PY - 2019
DA - 2019/05/10
PB - IJCSE, Indore, INDIA
SP - 25-30
IS - 01
VL - 06
SN - 2347-2693
ER -

Abstract :
Oral cavity is a vital part of the body and contributes to total health and wellbeing. Poor oral health affects general health and leads to systemic diseases like dental caries. The association between oral diseases and oral cavity flora is well established. There are more than 700 species of bacteria which may affect oral cavity and a number are implicated in oral diseases. Dental caries are developed by micro-organisms like Streptococcus, Lactobacillus and Actinomycetes which can be inhibited with the use of herbal and commercial mouthwashes as they show anti-microbial activity. Many researchers studied effect of anti-microbial activity of plant extracts on microbial flora of oral cavity. However, the studies on green synthesis of silver nanoparticles from the plant extracts and its effect on microbial flora of oral cavity would be a significant finding as these nanoparticles are efficient at very low concentrations and can penetrate into dental cavities easily. The silver nanoparticles play a crucial role in dental field for not only controlling the pathogens but also in other applications of nano dentistry. The present study includes screening, isolation and identification of microbial flora of oral cavity and to determine the effect of silver nanoparticles on oral flora. 50 oral cavity samples of patients fitted with dental braces from Army College of Dental Sciences and other dental clinics were collected. Streptococcus species was the predominant bacteria found in all these samples and the same organism is selected for comparative study of effect of commercially available mouthwashes like Orarite, Histidine and Whisper mint with that of herbal products like Azadirachta indica (Neem), Ocimum santum (Tulsi), Mentha (Peppermint), Piper betle (Betel) and Syzigium aromaticum (Clove). The results demonstrated that herbal products have more anti-microbial activity on Streptococcus species than the commercially available mouthwashes. These plant products were further tested for green synthesis of silver nanoparticles. The Silver Nanoparticles produced were characterized by Spectrophotometric, and SEM analysis. The silver nanoparticles synthesized from these plant products are safe, efficient and economical to use in herbal mouthwash preparations for dental diseases.

Key-Words / Index Term :
Green synthesis, Silver nanoparticles, Plant extracts, Oral flora

References :
[1]. Metwalli, K. H., Khan, S. A., Krom, B. P., & Jabra-Rizk, M. A. (2013). Streptococcus mutans, Candida albicans, and the Human Mouth: A Sticky Situation. PLoS Pathogens,9(10). doi:10.1371/journal.ppat.1003616
[2]. Palombo, E. A. (2011). Traditional Medicinal Plant Extracts and Natural Products with Activity against Oral Bacteria: Potential Application in the Prevention and Treatment of Oral Diseases. Evidence-Based Complementary and Alternative Medicine,2011, 1-15. doi:10.1093/ecam/nep067
[3]. Alshehri, F. A. (2018). The use of mouthwash containing essential oils (LISTERINE®) to improve oral health: A systematic review. The Saudi Dental Journal,30(1), 2-6. doi:10.1016/j.sdentj.2017.12.004
[4]. Babu, S. A., & Prabu, H. G. (2011). Synthesis of AgNPs using the extract of Calotropis procera flower at room temperature. Materials Letters,65(11), 1675-1677. doi:10.1016/j.matlet.2011.02.071
[5]. Salehi P., Momeni Danaie Sh. (2006). Comparison of the antibacterial effects of persica mouthwash with chlorhexidine on streptococcus mutans in orthodontic patients. Daru- Journal of Faculty of Pharmacy,14(4).
[6]. Shah, M., Fawcett, D., Sharma, S., Tripathy, S., & Poinern, G. (2015). Green Synthesis of Metallic Nanoparticles via Biological Entities. Materials,8(11), 7278-7308. doi:10.3390/ma8115377
[7]. Albandar, J. M., J. A. Brunelle, and A. Kingman. 1999. Destructive periodontal disease in adults 30 years of age and older in the United States, 1988–1994. J. Periodontol. 70:13–29.
[8]. Aas, J. A., Paster, B. J., Stokes, L. N., Olsen, I., & Dewhirst, F. E. (2005). Defining the Normal Bacterial Flora of the Oral Cavity. Journal of Clinical Microbiology,43(11), 5721-5732. doi:10.1128/jcm.43.11.5721-5732.2005
[9]. Gupta C, Kumari A, Garg, et al. A. Comparative study of cinnamon oil and clove oil in some oral microbiota. ABM [Internet]. 1Feb.2012 [cited 16Apr.2019];82(3):197-9.
[10]. Salehi P., Momeni Danaie Sh. (2006). Comparison of the antibacterial effects of persica mouthwash with chlorhexidine on streptococcus mutans in orthodontic patients. Daru- Journal of Faculty of Pharmacy,14(4).
[11]. Rosin M, Welk A, Bernhardt O, Ruhnau M, Pitten FA, Kocher T, Kramer A. Effect of a Polyhexamethylene Biguanide Mouthrinse on Bacterial Counts and Plaque. J Clin Periodontol, 2001; 28: 1121-1126.
[12]. Renton-Harper P, Addy M, Mora NJ, Doherty FM, Newcombe RG. A Comparison of Chlorhexidine, Cetylpyridinium Chloride, Triclosan and C31G Mouthrinse Products for Plaque Inhabitation. J Periodontol, 1996; 67: 486-89.
[13]. Almas K. The Effect of Salvadora Persica extract (Miswak) and chlorhexidine gluconate on human dentin: A SEM Study. J. Contemp. Dent. Pract. 2002; 3: 27-35.
[14]. Anderson G.B., Bowden J, Marrison E.C., Caffesse R. Clinical effect of chlorhexidine mouthwashes on patients undergoing orthodontic treatment. Am. J. Orthod Dentofac Orthop , 1997; 111: 606612.
[15]. Barrajo J.L., Varela L.G. Efficacy of chlorhexidine mouthrinses with and without alcohol: A clinical study. J Periodontol 2002; 73: 317-21.
[16]. Bishara S.E., Damon P.L., Olsen M.E., Jakobsen J.R. Effect of applying chlorhexidine antibacterial agent on the shear bond strengths of orthodontic brackets. Angle Orthod 1991; 66: 313-316.
[17]. Al-Otaibi M., Al-Harthy M., Gustafsson A., Classon R., Angmar-Mansson B. Subgingival plaque microbiota in Saudi Arabians after use of Miswak chewing stick and tooth brush. J. Clin. Periodontal. 2004; 31: 1048-53.
[18]. Thosar, Nilima, et al. “Antimicrobial Efficacy of Five Essential Oils against Oral Pathogens: An in Vitro Study.” European Journal of Dentistry, vol. 7, no. 5, 2013, p. 71., doi:10.4103/1305-7456.119078.
[19]. Malarkodi, C., et al. “Biosynthesis and Antimicrobial Activity of Semiconductor Nanoparticles against Oral Pathogens.” Bioinorganic Chemistry and Applications, vol. 2014, 2014, pp. 1–10., doi:10.1155/2014/347167.
[20]. Xi-Feng Zhang 1,Zhi-Guo Liu 1,Wei Shen 2 and Sangiliyandi Gurunathan 3,* Silver Nanoparticles: Synthesis, Characterization, Properties, Applications, and Therapeutic Approaches. Int. J. Mol. Sci. 2016, 17(9), 1534; https://doi.org/10.3390/ijms17091534
[21]. Saini, J., Kashyap, D., Batra, B., Kumar, S., Kumar, R., & Malik, D. K. (2011). Green Synthesis of Silver Nanoparticles by Using Neem (Azadirachta Indica) and Amla (Phyllanthus Emblica) Leaf Extract. Indian Journal of Applied Research,3(5), 209-210. doi:10.15373/2249555x/may2013/63
[22]. Bindhani, B. K., & Panigrahi, A. K. (2015). Biosynthesis and Characterization of Silver Nanoparticles (Snps) by using Leaf Extracts of Ocimum Sanctum L (Tulsi) and Study of its Antibacterial Activities. Journal of Nanomedicine & Nanotechnology,S6. doi:10.4172/2157-7439.s6-008
[23]. Gabriela, Á, Gabriela, M. D., Luis, A., Reinaldo, P., Michael, H., Rodolfo, G., & Roberto, V. J. (2017). Biosynthesis of Silver Nanoparticles Using Mint Leaf Extract (Mentha piperita) and Their Antibacterial Activity. Advanced Science, Engineering and Medicine,9(11), 914-923. doi:10.1166/asem.2017.2076
[24]. Bansal, H., Kaushal, J., Chahar, V., Shukla, G., & Bhatnagar, A. (2018). Green Synthesis of Silver Nanoparticles Using Syzygium aromaticum (Clove) Bud Extract: Reaction Optimization, Characterization and Antimicrobial Activity. Journal of Bionanoscience,12(3), 378-389. doi:10.1166/jbns.2018.1531
[25]. Rani, P. U., & Rajasekharreddy, P. (2011). Green synthesis of silver-protein (core–shell) nanoparticles using Piper betle L. leaf extract and its ecotoxicological studies on Daphnia magna. Colloids and Surfaces A: Physicochemical and Engineering Aspects,389(1-3), 188-194. doi:10.1016/j.colsurfa.2011.08.028
[26]. Botelho, M. A., Santos, R. A., Martins, J. G., Carvalho, C. O., Paz, M. C., Azenha, C., Ruela, F. I. (2009). Comparative effect of an essential oil mouthrinse on plaque, gingivitis and salivary Streptococcus mutans levels: A double blind randomized study. Phytotherapy Research, 23(9), 1214-1219. doi:10.1002/ptr.2489
[27]. Song, J. Y., & Kim, B. S. (2008). Rapid biological synthesis of silver nanoparticles using plant leaf extracts. Bioprocess and Biosystems Engineering, 32(1), 79-84. doi:10.1007/s00449-008-0224-6

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