Production of L-Arginine from arginine analogue resistant mutants of Corynebacterium glutamicum

Anjaneyulu Musini¹ , Lakshmi Narasu Mangamoori² , L. Saida³

1. Centre for Biotechnology, Institute of Science & Technology, Jawaharlal Nehru Technological University, Hyderabad, INDIA.
2. Centre for Biotechnology, Institute of Science & Technology, Jawaharlal Nehru Technological University, Hyderabad, INDIA.
3. Centre for Biotechnology, Institute of Science & Technology, Jawaharlal Nehru Technological University, Hyderabad, INDIA.

Section:Research Paper, Product Type: Isroset-Journal
Vol.5 , Issue.5 , pp.10-13, Oct-2018

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v5i5.1013

Online published on Oct 31, 2018

Copyright © Anjaneyulu Musini, Lakshmi Narasu Mangamoori, L. Saida . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
L-arginine is an essential amino acid, widely used in pharmaceutical, feed and food industry. It is the precursor molecule in citrulline and nitric oxide synthesis, which in turn plays significant role in a wide variety of patho - physiological functions in the cardiovascular, central and peripheral nervous systems. In the present study, mutagenesis experiments were carried out for isolation of analogue resistant mutants against Corynebacterium glutamicum. Initially, MIC (Minimum inhibitory concentration) was identified for arginine analogues by tube dilution method. MIC values found to be Arginine hydroximate – 680 µg/ml. Further, mutagenesis of C. glutamicum was carried out with NTG (300µg/ml) and amino acid analogue resistant mutants were isolated in minimal media. Fermentation was carried out by resistant colonies and estimated the Arginine levels. Production level was found to be 5.2 mg /ml where as in control cells it was 1.4 mg /ml. This study highlighted the C. glutamicum mutant of L-arginine amino acid production.

Key-Words / Index Term :
L-Arginine, Mutagenesis, Minimum inhibitory concentration and Corynebacterium glutamicum

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