In vitro Callus Induction and Preliminary Phytochemical Studies of Cissampelos pareira L

H.R. Raveesha¹ , T. Uthpala²

1. Department of Botany, Bangalore University, Jnanabharathi Campus, Bengaluru, India.
2. Department of Botany, Bangalore University, Jnanabharathi Campus, Bengaluru, India.

Correspondence should be addressed to: hrraveesh74@gmail.com.

Section:Research Paper, Product Type: Isroset-Journal
Vol.5 , Issue.1 , pp.12-17, Feb-2018

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v5i1.1217

Online published on Feb 28, 2018

Copyright © H.R. Raveesha, T. Uthpala . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
The present investigation was aimed to evaluate the effect of growth regulators on induction of callus from leaf segments of Cissampelos pareira. The surface sterilized young leaves were inoculated on Murashige and Skoog (MS) medium fortified with different concentration and combination of growth regulators for induction of callus. The phytochemical constituents were analyzed in the different solvent extracts using standard methods. Maximum callus induction was observed on MS medium supplemented with 2 mg/L of NAA or 2, 4-D alone and in combinations of NAA (2 mg/L) + BAP (1 mg/L) and NAA (2 mg/L) + KIN (0.5 mg/L). The preliminary phytochemical screening of leaf and leaf derived callus revealed the presence of flavonoids, alkaloids, phenols, terpenoids, coumarins etc. The developed protocol is useful for large scale production of callus from leaf explants which may be useful for isolation of important bioactive compounds for the treatment of various diseases.

Key-Words / Index Term :
Cissampelos pareira, callus, growth regulators, phytochemical screening

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